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The Microscopy Platform at The Children’s Hospital Research Institute of Manitoba offers a wide array of the latest technologies in biological imaging for cells and tissues, which are made available to both CHRIM scientists and other researchers on a user cost basis. The fee schedule is outlined below.
Access to this equipment requires in-person training. The minimum number of hours required for training is included in the fee schedule below. To inquire about training for any of the equipment listed here, contact the platform manager Gerald Stelmack at email@example.com or 204-789-3778. Users may not under any circumstances train each other. A user may require additional training sessions until a sufficient working knowledge can be demonstrated.
A trained CHRIM user will receive swipe card access to the microscope room and an online user account for booking the equipment. External users will not receive swipe card access and must be admitted by platform staff.
Once trained, a user should feel free to consult with technical staff if there is ANY doubt about using the equipment or software. Consultation will prevent damage and down time. Users must report problems promptly.
View the full platform SOP here.
Training Fee: hourly rate for one on one training
User Fee: hourly rate for equipment use by fully trained user
Full Service Fee: hourly rate for work done by technician (no training time required)
Consultation on what equipment is best suited for your study, some experiment planning, estimates of study time/costs, and technical support for a trained user are usually provided at no extra cost.
A laser scanning confocal microscope with spectral imaging capability. In addition to standard confocal microscopy, lambda scanning (spectral imaging) and post processing with linear unmixing algorithms allows for the separation of emission signals from overlapping fluorescent dyes and reduction or elimination of autofluorescent background signals.
Applications – Multicolor fluorescent imaging (up to 4 color channels) of immunocytochemically and immunohistochemically prepared specimens.
– Multidimensional image acquisition (time lapse and region of interest).
– Overlays of fluorescent and high contrast DIC (Nomarski) images.
– Optical sectioning (Z-stacking) with 3D image reconstruction.
– Spectral imaging with linear unmixing for separation of signals from dyes with overlapping fluorescent emissions.
– Long term live cell imaging using petri plates or chamber slides in a humidity, temperature, O2 and CO2 controlled environment.
– Protein kinetics (FRET, FRAP, Photoconversion and Photoactivation).
For details on instrument specifications, click here.
To book this microscope as a trained user, click here.
A laser scanning cytometer (LSC) for quantitative imaging cytometry. The LSC is a flow cytometer analogue adapted for both epifluorescent imaging and quantitative measurements of fluorescence in tissue sections and adherent cell systems. In addition, the system may be configured for quantitative analysis of chromatically stained tissue sections.
Applications – Quantitative fluorescence measurements in adherent cells and tissue sections.
– Quantitative nuclear fluorescence for analysis of cell cycle, DNA content, DNA damage, cell proliferation and apoptosis.
– Cell viability and immunophenotyping of cell populations.
– Analysis of cellular protein translocation and compartmentalization.
– Mitochondrial studies using Mitotracker and MitoSox dyes.
– Quantitative analysis chromatic stains in tissue sections.
– Quantitative analysis of viral infection or nucleic acid transfection efficiencies in cells.
For details on instrument specifications, click here.
An epifluorescence microscope configured for both fixed and live cell work.
Applications – Conventional epifluorescent imaging (photodocumentation) of immunocytochemically or immunohistochemically prepared specimens.
– Pecon stage insert incubator for long term live cell imaging using petri plates or chamber slides in a humidity, temperature, O2 and CO2 controlled environment.
A dual component system configured for both conventional epifluorescence microscopy and live cell calcium imaging using the calcium sensitive indicator dye, fura-2.
Applications – Conventional epifluorescent imaging (photodocumentation) of immunocytochemically and immunohistochemically prepared specimens.
– Long working distance 40x objective for short term fluorescent imaging of live cells through standard culture plates and culture clusters.
– NIS-Elements software includes a full suite of image processing and analysis tools and an additional plug-in module specific for image acquisition and analysis of data from live cell calcium imaging experiments.
An epifluorescence microscope system enclosed by an environmental chamber facilitating both a temperature and CO2 controlled environment for live cell imaging.
Applications – Live cell fluorescent imaging under a temperature and CO2 controlled environment.
–Conventional epifluorescent imaging of immunocytochemically and immunohistochemically prepared specimens.
-Custom filters for FRET using GFP/RFP1 and CFP/YFP donor/acceptor pairs.
An inverted research microscope coupled to video camera/video analysis software and an 8 channel perfusion valve control system.
Applications –The system is specifically configured for in-situ analysis of agonist induced changes in airway lumen diameter using vibratome sectioned lung tissue slices.